Description Oil Red O is a lysochrome diazo dye used for detecting neutral triglycerides and lipids in tissue. It is performed on fresh frozen tissue sections or cultured cells. Oil Red O Stain Kit is used for lipid and fat staining on formalin fixed frozen sections. It is not suitable for paraffin embedded tissue sections. VitroView™ Oil Red O Stain Kit and Protocol Video Kit Contents VB-3007-1 Pre-Stain Solution ———————– 100 ml VB-3007-2 Oil Red O Solution ————————100 ml VB-3007-3 Differentiation Solution————— 100 ml VB-3007-4 Mayer’s Hematoxylin Solution —- 100 ml Storage Room temperature. Protocol Cut frozen sections to 8 to10μm. Air dry the sections to the slides Fix in 10% buffered formalin for 20 mins at room temperature, briefly wash with running tap water for 3 mins. Place in Pre-Stain Solution for 2 mins Stain in pre-warmed Oil Red O solution for 10 minutes at room temperature Differentiate in Differentiation Solution for 5 minutes at room temperature Rinse in 2 water changes of distilled at room temperature. Lightly stain nuclei with Mayer’s Hematoxylin Solution for 1 minute. Rinse with tap water for 3 mins Mount with aqueous mounting medium. Technical Points Include a positive control Cryostat sections 8 to 10 microns for formalin fixed smears. Formalin fixation gives good results. Step 9 – Obviously, the section must not be taken through clearing solvents prior to mounting, as this will remove the lipid to be detected. Results Lipid…………………………….. red Nuclei…………………………….blue More Oil Red Staining Images Oil Red O Stain, Mouse Liver, 40x Oil Red O Stain, Mouse Liver, 20x Oil Red O Stain, Normal Mouse Liver, 20x Oil Red O Stain, Mouse Liver, 10x Oil Red O Stain, Mouse Liver-2, 20x Oil Red O Stain, Mouse Liver-3, 20x Note: This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals. Precautions: Handle with care. Avoid contact with eyes, skin and clothing. Do not ingest. Wear gloves. User Manual and Material Safety Data Sheet (MSDS) (PDF) VB-3007 User manual VB-3007 MSDS
