Description Congo Red is a histological stain that has been used to detect the presence of amyloid deposits, including those associated with Alzheimer’s disease. The stain binds to beta-pleated sheet structures, which are characteristic of amyloid fibrils. Therefore, Congo Red staining can be used to visualize beta-amyloid plaques in the brain tissue of individuals with Alzheimer’s disease. Congo Red Amyloid Stain Kit is used for the detection of amyloid in formalin-fixed, paraffin-embedded tissue sections with amyloidosis, and may be used for frozen sections as well. Amyloid deposits will stain red, while nuclei will stain blue. The thickness of sections is usually 5 µm. However, in cases containing low amyloid deposits, 10µm thick sections will give better results. Kit Contents VB-3011-1 Congo Red Solution ——————–250 ml VB-3011-2 Alkaline Alcohol Solution ————250 ml VB-3011-3 Mayer’s Hematoxylin Solution —- 250 ml Storage Condition: Store at room temperature. Protocol For paraffin-embedded tissue sections, deparaffinize and hydrate to 70% alcohol. Rinse in distilled water. For frozen sections, dry sections for 5 minutes. Fix sections in neutral formalin solution for 20 minutes. Rinse in distilled water. Stain in congo red solution for 15-20 minutes. Rinse in distilled water. Differentiate quickly (5-10 dips) in alkaline alcohol solution. Rinse in tap water for 1 minute. Counterstain with Mayer’s hematoxylin for 30 seconds. Rinse in tap water for 2 minutes. Dehydrate through 95% alcohol, 2 changes of 100% alcohol, 3 minutes each. Clear with 3 changes of xylene (5 minute per change) Mount coverslip onto glass slide with Permount or some other suitable organic mounting medium. Results Amyloid, elastic fibers, eosinophil granules ——– red Nuclei —————————————————————- blue Positive Controls Know amyloidosis containing tissue (i.e. Alzheimer’s disease). Note: This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals. Precautions: Handle with care. Avoid contact with eyes, skin and clothing. Do not ingest. Wear gloves. More Images Congo Red Amyloid Stain, Human Brain, Alzheimer’s disease, 4X Congo Red Amyloid Stain, Human Brain, Alzheimer’s disease, 20X Congo Red Amyloid Stain, Human Brain, Alzheimer’s disease, 20X Congo Red Amyloid Stain, Human Brain, Alzheimer’s disease, 40X Congo Red Amyloid Stain, Human Brain, Normal, 20X Congo Red Amyloid Stain, Blood Vessel in Mouse Lung, 20X User Manual and Material Safety Data Sheet (MSDS) (PDF) VB-3011 User manual VB-3011 MSDS
