Description The Verhoeff Elastic Masson Trichrome Stain offers a comprehensive method for simultaneously staining elastic, muscle, and connective tissue, making it ideal for routine use in histopathology. Verhoeff’s technique renders the elastica black, creating a distinct contrast with the red-stained muscle and green or blue-stained connective tissue, achieved through a modification of Masson’s trichrome. Additionally, cell nuclei exhibit reddish brown to blue-black hue with Weigert’s iron hematoxylin. This kit is particularly well-suited for examining vascular pathology in experimental pathology, surgical and autopsy sections. Kit Components Sku# Reagent Name Volume (ml) VB-3034-1 Ethanol saturated with picric acid 250 ml VB-3034-2 Verhoeff’s elastic stain solution A 150 ml VB-3034-3 Verhoeff’s elastic stain solution B 60 ml VB-3034-4 Verhoeff’s elastic stain solution C 60 ml VB-3034-5 Biebrich scarlet-acid fuchsin solution 250 ml VB-3034-6 Phosphotungstic/phosphomolybdic Acid Solution 250 ml VB-3034-7 Aniline Blue Solution 250 ml VB-3034-8 1 % acetic acid solution 250 ml Storage Store at room temperature. Protocol Deparaffinize in xylene (6 minutes) ×2 Rehydrate: Ethanol 100% (2 minutes) ×2; Ethanol 95% (2 minutes) ×2; Ethanol 70% (2 minutes) ×1. Rinse in distilled water (2 minutes) ×1. Mordant in ethanol saturated with picric acid for 5 minutes Wash sections in tap water for 5 min until yellow color disappears. Freshly prepare modified Verhoeff’s elastic stain solution: mix Verhoeff’s elastic stain solution A, B and C at a ratio of 2.5:1:1. Stain in modified Verhoeff’s elastic stain for 6 min. Wash in running tap water for 10 min. Rinse in distilled water for 1 min. Stain in Biebrich scarlet-acid fuchsin solution for 2-3 min Place in Phosphotungstic/phosphomolybdic Acid Solution for 15 min. discard solution. Transfer directly into Aniline Blue Solution for 5 minutes. 1% Acetic acid for 2-3 seconds discard solution. Dehydrate with 2 changes of 95% Ethanol and 2 changes of 100% Ethanol (2 minute per change). Clear with 2 changes of xylene (5 minute per change). Mount cover slip onto glass slide with Permount or some other suitable organic mounting medium. Control Tissue Artery or lung tissue Expected Results: Elastic fibers——————————blue-black Nuclei————————————–reddish brown to blue-black Muscle, keratin & cytoplasm ———-red Collagen———————————-green References: Garvey W, et al (1984).Modified elastic tissue-masson trichrome stain. Stain Technology. 59 (3): 213-216 Wu J, et al (2015). Full-Thickness Thermal Injury Delays Wound Closure in a Murine Model. Advances in Wound Care, 4(2):83-91 Note: This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals. Precautions: Handle with care. Avoid contact with eyes, skin and clothing. Do not ingest. Wear gloves. More Images Mouse Lung, Elastic Masson Trichrome Stain, 20x Mouse Bronchus, Elastic Masson Trichrome Stain, 20x Mouse Kidney, Elastic Masson Trichrome Stain, 40x User Manual and Material Safety Data Sheet (MSDS) (PDF) VB-3034-User Manual VB-3034 MSDS
